Effects of p21 gene transfection on the growth and cisplatin sensitivity of cervical cancers cell line Hela

Objective To investigate the effects of human p21 WAF1/CIP1(p21 gene)transfection on the growth and chemosensitivity of human cervical carcinoma cell line hela.Methods Recombined vector pcDNA3 were transferred to Hela cell line by Lipofection.These two cell lines were selected by G418,and the selected cell lines were subcultured.Hela cell line were enclosed as control group and the Neutral red was used to explore the inhibitory effect of chemotherapeutic drugs on human Hela cell transfected with p21 gene and the changes of cell cycle and cell apoptosis rate were detected by flow cytometry.Results After G418 selecting,Hela cell line in control group was died,and the cell line transferring pcDNA3 were subcultured.After transfection of p21 gene,cell growth was obviously inhibited and resulted in an accumulation of cell in G1 phase,presenting that the proportion of the cell in G1 phase was obviously increased and that of the cells in S and G2/M phases was obviously decreased.Respectively as compared with control group.The apoptotic index was increased in Hela-p21 cell.p21-positive Hela was more sensitive to Cisptatin.Conclusion The exogenous p21 gene expressions not only induced great suppression of cell growth but also increased chemosensitivity of human cervical carcinoma.