The Relationship between VEGF in Experimental Rat and Tumor Angiogenesis Invasion and Metastasis

Objective To investigate the expression of serum vascular endothelial growth factor(VEGF) in experimental rat.Methods Measured serum vegf concentration by ELISA and vegf expression in tumor tissue by immunohistochemical staining in experimental rat.Factor Ⅷ was immunohistochemicaly used to count microvascular density in tumor tissue.Results We found that sVEGF concentrations in experimental rat distinctly rised after tumor cells transplanted in the rat's spleen and the level of vegf were stable in that two weeks.The expression of vegf in experimental rat was closely correlated with microvascular density(MVD),γ =0.92,P 0.05.VEGF expression in cell plasma detected by immunohistochemistry,its expression(2.57 ± 0.68 ng/ml) in high level hepatic metastasis was stronger than that(1.83 ± 0.37 ng/ml) in low or negtive metastasis(P 0.05).The concentrations of serum vegf in experimental rat with peritoneal spreading and bloody ascites were higher than that with not(2.47 ± 0.71 ng/ml vs 1.86 ± 0.68 ng/ml,2.47 ± 0.66 ng/ml vs 1.75 ± 0.38 ng/ml,P 0.05).Conclusion The results indicted that the expression of vegf in experimental rat is closely correlated with angiogenesis,invasion and metastasis of tumor