Rapid detection of Escherichia Coli O157:H7 in meat using an improved loop-mediated isothermal amplification technology

The detection period of Escherichia coli O157: H7(E.coli O157: H7) using traditional test-ing methods is relatively long.Therefore a kind of rapid detection method,namely a loop-mediated isothermal amplification(LAMP) technology with two loop primers detection E.coli O157: H7 in meat,was developed to address this problem.Two sets LAMP primers with loop primers were respectively designed based on the target sequences of rfbE and fliC genes of E.coli O157: H7.The detection results were judged by observing the white precipitate at simultaneous detection of single-tube.The specificity of the improved LAMP was verified using 36 strains of bacteria.DNA extracted by pyrolysis was am-plified 20 min by the LAMP system.The detecting sensitivity of E.coli O157: H7 was 1.4 CFU/mL.The detection limit of E.coli O157: H7 from artificial contamination meat was 1.8 CFU/g.One false positive of 137 meat samples was detected,which can obtain compliance rate of 99.3% with the indus-try standard SNT0973-2000.