Effects of Siwu Decoction with Different Extracting Techniques on Hmatopoietic Function in Mice with Blood Deficiency

Objective:To study the effects of Siwu decoction with different extracting techniques including water extract,water extract and alcohol precipitate,and 50% ethanol percolation on hematopoietic function in mice with blood deficiency.Method: The mice were randomly divided into six groups: normal gruop,model group,positive group,water extract group,water extract and alcohol precipitate group,50% ethanol percolates group.The Siwu decoction groups were treated with the dosage of 12 g·kg-1,positive group was goven Fufang E-Jiao Jiang 20 mL·kg-1,normal group and model group were given physiological saline,fro 8 days.Except normal group,every group received subcutaneous injection with APH in the second and fifth day with the dosage of 20,40 mg·kg-1 respectively.From the fifth day,every group received peritoneal injection with CTX 40 mg·kg-1 every day for four days.The normal group was given physiological saline.Then,the peripheral hemogram,index of spleen and thymus,blood monomuclear cell(BMC) were detected in different groups.Result: All Siwu decocotion groups could inhance the number of red blood cell(RBC),white blood cell(WBC),BMC and the content of HGB,and the effects of water extract group and 50% ethanol percolates group were more obvious(P0.01) meanwhile Siwu decocotion groups could increase index of spleen and thymus(P0.01).Conclusion: Siwu decoction can improve the hematopoietic function in mice with blood deficiency,and the water extract group and 50% ethanol percolates group were better than water extract and alcohol precipitate group.