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2004•Journal of Clinical Laboratory ScienceRequires access

Detection of extended-spectrum beta-lactamases produced from Klebsiella pneumoniae

Ming Liu

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Abstract

Objective To investigate the production of extended-spectrum beta-lactamases (ESBLs) in Klebsiella pneumoniae.Methods ESBLs of Klebsiella were screened by double disk diffusion test and E test.SHV beta-lactamase gene was detected by SHV-PCR, and SHV-ESBL were further examined by PCR-NheⅠmethod.Results Among 36 strains of Klebsiella pneumoniae 30 strains produced beta-lactamases, 31 strains contained SHV beta-lactamase genes, 8 strains were positive ESBLs phenotype and 3 strains were SHV-ESBLs.Conclusion Klebsiella pneumoniae is one of the most common species which produce ESBLs.More attention should be paid to its detection and surveillance.

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What this paper is about

Objective To investigate the production of extended-spectrum beta-lactamases (ESBLs) in Klebsiella pneumoniae.Methods ESBLs of Klebsiella were screened by double disk diffusion test and E test.SHV beta-lactamase gene was detected by SHV-PCR, and SHV-ESBL were further examined by PCR-NheⅠmethod.Results Among 36 strains of Klebsiella pneumoniae 30 strains produced beta-lactamases, 31 strains contained SHV beta-lactamase genes, 8 strains were positive ESBLs phenotype and 3 strains were SHV-ESBLs.Conclusion Klebsiella pneumoniae is one of the most common species which produce ESBLs.More attention should be paid to its detection and surveillance.

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Available abstract

Objective To investigate the production of extended-spectrum beta-lactamases (ESBLs) in Klebsiella pneumoniae.Methods ESBLs of Klebsiella were screened by double disk diffusion test and E test.SHV beta-lactamase gene was detected by SHV-PCR, and SHV-ESBL were further examined by PCR-NheⅠmethod.Results Among 36 strains of Klebsiella pneumoniae 30 strains produced beta-lactamases, 31 strains contained SHV beta-lactamase genes, 8 strains were positive ESBLs phenotype and 3 strains were SHV-ESBLs.Conclusion Klebsiella pneumoniae is one of the most common species which produce ESBLs.More attention should be paid to its detection and surveillance.

Key concepts: Klebsiella pneumoniae, Microbiology, Klebsiella, Beta-lactamase, Biology, Enterobacteriaceae, Gene, Escherichia coli

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