Establishment and application of ELISA for diagnosis of bovine schistosomiasis using Schistosoma japonicum bivalent epitope antigen

In order to establish a sensitive and specific method for diagnosis of schistosomiasis,the expression and purification of Schistosoma japonicum bivalent epitope antigen(pGEX-BSjGCP-BSj23) was carried out.Using the recombinant protein(10 mg/L) and 1∶40 000 diluted HRP rabbit anti-bovine IgG,an indirect ELISA was developed for diagnosis of bovine schistosomiasis.SEA antigen was used as a control to compare the sensitivity and specificity of the developed ELISA.In result,the bivalent epitope antigen based ELISA was significantly more specific than the method based on SEA,and was similar in sensitivity.Regarding the buffalo sera detected by the developed ELISA,the negative coincidence rate was 100% and positive coincidence rate was 90.97%.The result showed that the developed ELISA was specific and sensitive for diagnosis of bovine schistosomiasis.