Changes of renal AQP3 expression in rats after recanalization of bile duct occlusion

Objective To explore the changes of renal AQP3 mRNA in rats after recanalization of bile duct occlusion. Methods The kidney samples used in this study were from the previous experiment, so were the biochemical parameters. The expression of AQP3 mRNA was detected by RT-PCR. Results Levels of TBIL and ALT were decreased gradually with the increasing duration of recanalization of the occluded bile duct (P0.05). As compared with the control subjects, levels of BUN and Cr did not differ significantly in the rats at the moment their bile duct was recanalized (P0.05), AQP3 mRNA expression was downregulated (P 0.05); levels of BUN and Cr were increased on day 1 of recanalization (P0.05) and AQP3 mRNA expression was further declined (P0.05); on day 3, levels of BUN and Cr did not differ significantly from those on day 1 (P0.05), and AQP3 mRNA expression was upregulated but the expression level was lower (P0.05); on day 7, there were no significant differences in levels of BUN and Cr and AQP3 mRNA expression. Conclusions Following recanalization of bile duct occlusion, the change in AQP3 mRNA expression is similar to that in AQP3.