2015•Guoji yaoxue yanjiu zazhiRequires access

Application and prospect of nucleic acids detection for Ebola virus

Jia Ji

Abstract

Ebola virus(EBOV)causes severe hemorrhagic fever in humans and nonhuman primates with high mortality rates.Zaire ebola virus is the cause of the 2013-2014 Ebola virus epidemic in West Africa. Rapid identification of the virus is urgently required to prevent the spread of the infection. The real-time qutification PCR assay and the loop-mediated isothermal amplification assay(LAMP) have been developed for EBOV detection; the nucleic acid sequence-based amplification assay(NASBA) and the recombinase polymerase amplification assay(RPA) have great potential for diagnosis of EBOV in the field. This review highlights various methods that can be used for a sensitive detection of EBOV nucleic acids.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Ebola virus, Recombinase Polymerase Amplification, NASBA, Virology, Nucleic acid, Loop-mediated isothermal amplification, Biology, Virus