The effects of mesenchymal stem cells transplantation on the brain-derived neurotrophic factors after the spinal cord injury

Objective To observe the effects of mesenchymal stem cells(MSCS)transplantation on the brain-derived neurotrophic factors(BDNF)after the spinal cord injury(SCI)of rats,and to investigate the mechanism of repairing the SCI by MSCS transplantation.Methods Mesenchymal stem cell were cultured from the femur of adult SD rats and identified by immunohistochemistry.Seven days after the operation of SCI,the mesenchymal stem cells were transplanted into the injured spinal cord,sixty adult SD rats were randomly divided into three groups:(group A)SCI cured with transplantation of mesenchymal stem cells;(group B)SCI received PBS solution,(group C)control group.Then the expression of brain-derived neurotrophic factors of the lesion and neighbour areas were examined through immunohistochemistry.Investigated the mechanism of repairing of the lesion and neighbor areas,and the mechanism of repairing the spinal cord after mesenchymal stem cells transplantation.Results Transplantation of mesenchymal stem cells enhanced the expression of brain-derived neurotrophic factors.There are significant difference among group A,B and C.Conclusion The expression of brain-derived neurotrophic factors in group A showed a higher level with a long time lasting than group B.It maybe one of the mechanisms of repairing the SCI by mesenchymal stem cells transplantation.