Extraction and determination of cordyceps acid in submerged fermentation mycelium of Cordyceps Militaris strains

Cordyceps acid,that is,D-mannitol,is one of the main active ingredients in the type of fungus cordyceps.To explore Cordyceps militaris better,the extracting technology of cordyceps acid in several strains of Cordyceps militaris was determined.According to a large number of single-factor experiments and orthogonal design,the best extraction conditions were drawn:microwave time 80s,microwave power 200W,material-liquid ratio 1:100,ethanol solvent concertration 70%.The strain with the highest acid content among 20 kinds of Cordyceps militaris strains was No.22 strain,whose cordyceps acid content was 160.39mg/g,which had value in production and application.