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2013•Guangdong nongye kexueOpen access

Effect of Water Copper on Content of Hepatopancreas CP mRNA in Cyprinus carpio

Chunsheng Gao

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Abstract

In order to explore the effect of water copper on the content of hepatopancreas CP mRNA in Cyprinus carpio,some Cyprinus carpio were stimulated by 0,0.21,0.30,0.43,0.60,0.81 mg / L of Cu2 + at the same time,and on the 2,4,6,8,10th day after treatment,the contents of hepatopancreas CP mRNA were respectively monitored by using semi-quantitative RTPCR method.The results indicated that: under the simulation of different concentrations of copper in water body,the expression of CP mRNA in the liver and pancreas of Cyprinus carpio showed a time dependence and a dose dependence.Along with the change of the time,CP mRNA expression at first increased and then decreased;the CP mRNA expression could reach a peak,but the peak time was not the same.

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What this paper is about

In order to explore the effect of water copper on the content of hepatopancreas CP mRNA in Cyprinus carpio,some Cyprinus carpio were stimulated by 0,0.21,0.30,0.43,0.60,0.81 mg / L of Cu2 + at the same time,and on the 2,4,6,8,10th day after treatment,the contents of hepatopancreas CP mRNA were respectively monitored by using semi-quantitative RTPCR method.The results indicated that: under the simulation of different concentrations of copper in water body,the expression of CP mRNA in the liver and pancreas of Cyprinus carpio showed a time dependence and a dose dependence.Along with the change of the time,CP mRNA expression at first increased and then decreased;the CP mRNA expression could reach a peak,but the peak time was not the same.

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Available abstract

In order to explore the effect of water copper on the content of hepatopancreas CP mRNA in Cyprinus carpio,some Cyprinus carpio were stimulated by 0,0.21,0.30,0.43,0.60,0.81 mg / L of Cu2 + at the same time,and on the 2,4,6,8,10th day after treatment,the contents of hepatopancreas CP mRNA were respectively monitored by using semi-quantitative RTPCR method.The results indicated that: under the simulation of different concentrations of copper in water body,the expression of CP mRNA in the liver and pancreas of Cyprinus carpio showed a time dependence and a dose dependence.Along with the change of the time,CP mRNA expression at first increased and then decreased;the CP mRNA expression could reach a peak,but the peak time was not the same.

Key concepts: Cyprinus, Hepatopancreas, Messenger RNA, Copper, Chemistry, Real-time polymerase chain reaction, Biology, Endocrinology

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Effect of Water Copper on Content of Hepatopancreas CP mRNA in Cyprinus carpio — Research Paper | ScholarLens