Expression of Skp2 protein in non-small cell lung cancer and its relationship with p27~(kip1) protein expression

Objective To investigate the expression of Skp2 and its relationship with expression of p27~(kip1) protein in human non-small cell lung cancer(NSCLC).Methods Immunohistochemical method was applied to detect the expression of Skp2,p27~(kip1) proteins in 60 surgical specimens from NSCLC patients and 20 normal bronchial epithelium.Results In NSCLC,the positive rate of Skp2 protein was 48.33%(29/60).Expression of Skp2 protein was detected in a significantly greater proportion in NSCLC than that in normal bronchial epithelium(P0.01);There was no relationship between Skp2 expression and age or sex;otherwise,there was closely relationship between Skp2 expression and histological subtype,cellular differentiation,lymph node metastases and smoking;Expression of Skp2 was negatively correlated with expression of p27~(kip1)(P0.01).Conclusion Expression of Skp2 protein reduced the protein level of p27~(kip1),indicating Skp2 played an important role in oncogenesis and development of NSCLC.