The Effect of Oxidative Stress on Myocardial Injury in Rats with Sepsis

Objective To investigate the pathological changes and alterations of parameters associated with oxidative stress in myocardium of rats with sepsis.Methods Thirty-eight Wistar rats were randomly divided into two groups: sepsis model group(CLP group: cecal ligation and puncture to establish sepsis model,n=25) and control group(sham procedure,n=13).24 hours later after procedure,pathologic changes in heart tissue of all animals were observed.Meanwhile serum concentrations of myocardial enzyme and cardiac superoxide dismutase(SOD),glutathione peroxidase(GSH-PX) and malondialdehyde(MDA) were detected in both groups, the capability of inhibition of superoxide anion,hydroxyl radicals in the heart tissue were also measured.Results The pathologic changes of heart tissue in septic rats included edema,congestion and inflammatory cells infiltration in mesenchyma,no pathological damage was seen in control group rats.Compared with controls,serum concentration of myocardial enzyme: cardiac SOD,GSH-PX,MDA and the capability of inhibition of superoxide anion were significantly increased in septic rats 24 hours post-operation(P0.01).Conclusions Oxidative stress involves in the pathogenesis of the myocardial injury in sepsis.