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2008•Journal of the South China Agricultural UniversityRequires access

Cloning and Expression of Hai-Lan Chicken Interleukin-18 in E.coli and Preparation of Its Antiseru

Zheng LanLan

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Abstract

Chicken interleukin-18(ChIL-18) mature protein gene was amplified from PHA stimulated Hai-Lan chicken spleen cell by RT-PCR.PCR product was cloned into the pGEM-T vector.Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0 bp including the stop coden and,pQE30-ChIL18 was obtained by subcloning the encoding region of the ChIL-18 mature peptide gene into pQE30.The recombinant ChIL-18(rChIL-18) was expressed efficiently in pQE30-ChIL18-transformed M15 induced by IPTG.SDS-PAGE analysis showed that the recombinant mature protein had a relative molecular mass of approximately 19 000.

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What this paper is about

Chicken interleukin-18(ChIL-18) mature protein gene was amplified from PHA stimulated Hai-Lan chicken spleen cell by RT-PCR.PCR product was cloned into the pGEM-T vector.Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0 bp including the stop coden and,pQE30-ChIL18 was obtained by subcloning the encoding region of the ChIL-18 mature peptide gene into pQE30.The recombinant ChIL-18(rChIL-18) was expressed efficiently in pQE30-ChIL18-transformed M15 induced by IPTG.SDS-PAGE analysis showed that the recombinant mature protein had a relative molecular mass of approximately 19 000.

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Available abstract

Chicken interleukin-18(ChIL-18) mature protein gene was amplified from PHA stimulated Hai-Lan chicken spleen cell by RT-PCR.PCR product was cloned into the pGEM-T vector.Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0 bp including the stop coden and,pQE30-ChIL18 was obtained by subcloning the encoding region of the ChIL-18 mature peptide gene into pQE30.The recombinant ChIL-18(rChIL-18) was expressed efficiently in pQE30-ChIL18-transformed M15 induced by IPTG.SDS-PAGE analysis showed that the recombinant mature protein had a relative molecular mass of approximately 19 000.

Key concepts: Subcloning, Molecular biology, Recombinant DNA, Biology, Gene, Cloning (programming), Sequence analysis, lac operon

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Cloning and Expression of Hai-Lan Chicken Interleukin-18 in E.coli and Preparation of Its Antiseru — Research Paper | ScholarLens