Construction of bladder carcinoma cells transducted by IL-18 and study of its antitumor effect

Objective To set up bladder carcinoma cells(T24) transducted by IL-18,and study its antitumor effect and related mechanism.Methods Transducted T24 cell with the culture supernatant of IL-18/PA317 cells and gained IL-18/T24 cells selected with G418.IL-18 mRNA expression in IL-18/T24 cells was detected with RT-PCR.The expression of IL-18 protein was detected by ELISA.The proliferation of T24,LXSN/T24 and IL-18/T24 cells were detected by MTT colorimetry in vitro.Observe the growth of transplanted tumor in nude mouse.Flow cytometry was used to analyze the cell apoptosis.Results IL-18/T24 cells expressing IL-18 stably was successfully set up.The proliferation rate of IL-18/T24 cells showed no difference with parental cells,and the cell apoptosis of IL-18/T24 cells was similar in vitro(P0.05).Compared with the control group,growth rate of tumor in mice model was inhibited and cell apoptosis rate was increased significantly(P0.01) in IL-18 gene therapy group.Conclusion The cellular clone IL-18/T24 with high level expression of IL-18 was produced and IL-18 has no effect on cell apoptosis in vitro,while it played a role in antitumor immunity by inducing cell apoptosis in vivo.