2014•Journal of Shandong UniversityRequires access

Protective effect of autophagy on the inflammation induced by ox-LDL

Hao Fengchen

Abstract

Objective To study the effect of autophagy on the inflammation induced by oxygenized low density lipoprotein( ox-LDL). Methods The THP1 cells w ere divided into four groups: control group,ox-LDL treatment group, 3-methyladenine( 3-MA) treatment group,ox-LDL combined w ith 3-MA treatment group. The expression of LC3 protein w as detected by Western blotting. The formation of autophagosome w as measured by immunofluorescence w ith LC3-specific antibody. MDC staining w as used to detect the intracellular acidic vesicles. The changes of inflammatory factor level w as determined by ELISA analysis. Results Ox-LDL could induce autophagy in monocyte THP1. The inflammatory reaction induced by ox-LDL was significantly enhanced after inhibition of autophagy( P 0. 05). Conclusion Autophagy represents a defense mechanism in the inflammation induced by ox-LDL.

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Key concepts: Autophagy, Inflammation, Chemistry, Intracellular, Blot, Low-density lipoprotein, Monocyte, Immunofluorescence