Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances

Objective To explore the expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9)in the tumor tissue of the patients with esophageal squamous cell carcinoma,and to analyze the correlation between the expressions of MMP-2and MMP-9.Methods 100 spicemens of surgical removed esophageal squamous cell carcinoma were chosen.The tumor tissue and the adjacent normal esophagel mucosa tissue were selected.RT-PCR method was used to detect the expressions of MMP-2and MMP-9mRNA in the esophageal squamous cell carcinoma tissue and the adjacent normal tissue.Western blotting method was used to testify the expressions of MMP-2and MMP-9proteins;the correlation between the expressions of MMP-2and MMP-9was analyzed by Pearson correlation analysis.Results The positive expression rate of MMP-2 mRNA in carcinoma tissue was 89.0%(89/100),the positive expression rate of MMP-2mRNA in adjacent normal tissue was76.0%(76/100),and there was statistical difference between two groups(χ2=5.853,P =0.016).The positive expression rate of MMP-9mRNA in carcinoma tissue was 48.0%(48/100),the positive expression rate in adjacent normal tissue was 16.0%(16/100),and there was statistical difference of the positive expression rate between two groups(χ2=23.529,P=0.000).There was a positive correlation between the expression of MMP-2mRNA and the tumor invasion depth(χ2=4.971,P=0.026),but there was no correlation between the expression of MMP-2mRNA and the lymph node metastasis(χ2=2.127,P=0.145).There was a positive correlation between the expression of MMP-9mRNA and the tumor invasion depth(χ2=4.421,P=0.035),but there was no correlation between the expression of MMP-9mRNA and lymph node metastasis(χ2=0.617,P=0.432).There was positive correlationship between the positive expression rates of MMP-2 mRNA and MMP-9 mRNA in esophageal cancer tissue(r=0.274,P=0.006).Conclusion The MMP-2and MMP-9may play a very important role in the occurrence and development of esophageal squamous cell carcinoma,however they have no affection on lymph node metastasis of esophageal squamous cell carcinoma.