Construction of retrovirus vector for human p27~(kip1) gene and its inhibitory effect on human gastric cell line

Objective:To study the inhibitory effects of retro virus mediated p27 kip1 gene on cell cycle and proliferation.Methods:Recombinant retrovirus eukaryotic expression vector pLNCX p27 kip1 containing exogenous human p27 kip1 cDNA was constructed and used to infect cells.The exogenous p27 kip1 expression in BGC 823 cells and its effects on proliferation were determined by Western blot,FCM staining.Results:Exogenous p27 kip1 had successfully been transferred into BGC 823 cells and obtained permanent expression.The growth rate of these transfected BGC 823 cells was inhibited.The percentage of phase G 1 cells was increased and that of phase S cells was decreased by analyzing cell cycle.Conclusion:p27 kip1 recombinant retrovirus prepared in this study can efficiently transfer p27 kip1 into gastric cell line,and the over expression of p27 kip1 inhibits the proliferation,suggesting that p27 kip1 might be used as a potential therapeutic agent for the gene therapy on human gastric cancer.