Studies on the Resonance Rayleigh Scattering of Methyl-violet-Chondroitin Sulfate Complex and Its Analytical Application

A simple and rapid method for the determination of chondroitin sulfate(CS) was developed.It is based on the enhancement of the intensity of resonance Rayleigh scattering(RRS) resulted from the ion-association complex formed between methyl violet(MV) and chondroitin sulfate(CS) in Britton-Robinson(B-R) buffer(pH 9.37).The maximum RRS peaks were at the wavelengths of 505 nm and 661 nm,respectively.The optimum conditions for the MV-CS interaction and the relationship between the relative intensity of RRS and the concentration of CS have been investigated in detail.The linear ranges of the calibration curve for CS at 505 nm and 661 nm were both 0.15-0.90 mg/L.The detection limits were 0.019 mg/L at 505 nm and 0.043 mg/L at 661 nm,respectively.It has been applied to the determination of CS in chondroitin sulfate injection samples with satisfactory results.