Survivin gene silencing inhibited growth of colon cancer Lovo cells in vitro

Objective To investigate the inhibitory effect of surviving-shRNA on survivin gene expression and cell proliferation of the human colon cancer cell.Methods The survivin-shRNA was constructed and transfected into Lovo cells with Lipofectamine.RT-PCR and western blot analysis were used to detect down-regulations of survivin mRNA and protein expressions respectively.Cell proliferation inhibition was determined using MTT assay.Results The expressions of survivin mRNA and protein were successfully suppressed by survivin-shRNA(P0.05),with inhibition rates of 39.59% and 42.32% respectively.MTT assay indicated that the specific shRNA inhibited tumor cell growth in a time-dependent manner since 48 hours post-transfection.The cell viability of surviving-shRNA transfected group decreased significantly to 41.8% at 72 h,which was of statistical significance compared with that of blank control group(P0.05).Conclusion The specific survivin-shRNA could down-regulate the expression of survivin gene and inhibit the growth of Lovo cells in vitro.[