Study on Enzymatic Extraction of Anthocyanins from Blackcurrant Marc
Miao Yu
Abstract
Miao Yu
Abstract
Through experiments,the optimum conditions of enzymatic extraction of anthocyanin from blackcurrant(Ribes nigrum L.)marc,using cellulase were as follows:temperature 50 ℃,time 100 min,ratio of marc to solvent 1 ∶9,amount of enzyme 1 % of material,pH value of enzyme solution 5.0,the extraction rate of anthocyanin was 86.63 %.Using pectinase,the optimum conditions were:temperature 50 ℃,time 120 min,ratio of marc to solvent 1 ∶8,amount of enzyme 1.4 % of material,pH value of enzyme solution 3.5,the extraction rate of anthocyanin was 91.09 %.The extraction effect of pectinase is better than that of cellulase.
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Through experiments,the optimum conditions of enzymatic extraction of anthocyanin from blackcurrant(Ribes nigrum L.)marc,using cellulase were as follows:temperature 50 ℃,time 100 min,ratio of marc to solvent 1 ∶9,amount of enzyme 1 % of material,pH value of enzyme solution 5.0,the extraction rate of anthocyanin was 86.63 %.Using pectinase,the optimum conditions were:temperature 50 ℃,time 120 min,ratio of marc to solvent 1 ∶8,amount of enzyme 1.4 % of material,pH value of enzyme solution 3.5,the extraction rate of anthocyanin was 91.09 %.The extraction effect of pectinase is better than that of cellulase.
Key concepts: Pectinase, Anthocyanin, Chemistry, Cellulase, Extraction (chemistry), Ribes, Enzyme, Chromatography