Preparation and Characterization of Gentiopicroside Nano-lipid Carriers

Objective: To prepare gentiopicroside nano-lipid carriers(GEN-NLC) and investigate its stability,in vitro release and properties.Method: Solvent dispersion method was used to prepare GEN-NLC.With particle size,Zeta potential and encapsulation efficiency as indexes,effects of proportion of solid-liquid lipid,drug-lipid ratio and the type and concentration of surfactant on preparation technology were investigated,morphology of prepared GEN-NLC was observed by transmission electron microscopy,its characteristics and stability was investigated.Result: Optimal prescription was as following: 0.1% poloxamer 188 as surfactant,drug-lipid ratio 1 ∶ 10,liquid lipid ratio 10%.Encapsulation efficiency of prepared GEN-NLC was(38.19 ± 1.61) %,drug loading was(3.47 ± 0.08) %,particle size was(129.9 ± 3.07) nm,PDI was(0.264 ± 0.01),Zeta potential was(-22.5 ± 0.42) mV.GEN-NLC was spherical particles and showed monodisperse distribution,rounded appearance and uniform size.After stored at 4 ℃ for 30 d,indicators were not changed significantly,release of gentiopicroside 39.65% in 4 h,cumulative release amounted to 79.86% at 36 h.Conclusion: It showed that there were good physicochemical properties and stability of GEN-NLC prepared by solvent dispersion method,and had a certain degree of sustained-release long-lasting effect.