Expression of hippocampus of glycogen synthase kinase-3β and transforming growth factor-β1 in rats with chronic heart failure

Objectives To investigate the expression of hippocampus of glycogen synthase kinase-3β(GSK-3β) and transforming growth factor-β1(TGF-β1) in rats with chronic heart failure.Methods Totally 25 adult male SD rats were randomly assigned to 3 groups : normal group(n=5),sham operation group(n=10) and model group(n=10).Observation time of sham operation group and model group was 1 week,2 weeks,4 weeks and 8 weeks after operation.Chronic heart failure model was established by abdominal aortic constriction.Left ventricular end-diastolic dimension(LVEDd),interventricular septal thickness(IVST) and left ventricular posterior wall thickness(LVPWT) were monitored by ultrasound cardiography.Cardiac mass index,GSK-3β and TGF-β protein expression in hippocampal area were measured.Results LVEDd,IVST and LVPWT of model group 1 week after operation were(4.87±0.26) mm,(2.04± 0.11) mm and(1.93±0.19) mm respectively,and they showed an increasing tendency afterwards,reaching to(6.94± 0.12) mm,(2.98 ±0.21) mm and(3.63 ±0.18) mm 8 weeks after operation,significantly higher than those of normal group and sham operation group(P 0.01).LVEDd,IVST and LVPWT of sham operation group had no significant differences at different time points(P0.05).Cardiac mass index and left ventricular mass index of model group were significantly higher than those of normal group and sham operation group(P0.01).Western blotting values of GSK-3β among normal group,sham operation group and model group had no significant differences(P 0.01).Western blotting value of p-GSK-3β in model group 8 weeks after operation was significantly higher than those of normal group and sham operation group(P0.05).Express of TGF-β1 of model group was significantly higher than those of normal group and sham operation group(P 0.05)..Linear regression analysis indicated that expressions of p-GSK-3β and TGF-β1 had linear correlations with left ventricular mass index.Conclusions Proteins of p-GSK-3β and TGF-β1 in the hippocampus increase during the pathophysiologic processes of heart failure.