Effect of Atrazine on Micronucleus Rates of Bone Marrow and Histological Change of Kedney and Spleen in Male Rats

The purpose of this study was to investigate the effect of atrazine on the micronucleus rates of bone marrow and the histological changes of kidney and spleen in male rats.Four-week-old 60 male rats were randomly divided into 4 groups(control,low,middle,and high dose groups),15 in each group.In dose groups,rats were afforded atrazine by intraperitoneal injection according to 1/32 LD50(55.625mg),1/16 LD50(111.5mg) and 1/8 LD50(225.5mg) per kg weight on 0 day,30d,60d,respectively.The rats were killed at the 30d,60d and 90d,then bone marrow,kidney and spleen tissue samples were collected to detect the bone marrow micronucleus rates and histological changes of kidney and spleen.The results showed that the application of atrazine for longer time,micromucleus rates of bone marrow increased.Low,medium,high dose groups' micromucleus rates were respectively 17.14%,133.33%,119.52% higher than that of the control group.The high dose group was the highest in 60d,which was respectively higher 118.65%,55.10% than that of 30d and 90d.The kidney tissue presented proliferative and degenerative changes,spleen tissue presented fatty degeneration,which showed that the application prolonged of atrazine may lead to male rats appeared certain genetic toxicity and kidney and spleen tissue lesions.