Studies on Somatic Embryogenesis and Cell Morphology of Immature Asparagus officinalis L.Embryos

Three varieties(C.IL,PURPLE and APOLLO) were applied in somatic embryogenesis of Asparagus officialis L.Different growth substances such as 2,4-D,KT,6-BA and NAA were added in the MS substrate.Results indicated that somatic embryos were induced directly from immature embryo axes cultured on basal MS medium supplemented with 2,4-D 1.0 mg/L,KT 0.5 mg/L and 6-BA 2.0 mg/L.When decreasing the level of 2,4-D,yellow embryogenic callus were induced from tissue of somatic embryos cultured for 2-3 times,and somatic embryos at different stages appeared.The embryogenic cell first divided into multicellular proembryos,then globular embryoid,pearshaped embryoid,long embryoid,scutiform embryoid and mature embryoid.Asparagus officialis L.embryogenesis from epidermical cells or subepidermical cells was similar to that of the zygotic embryo.