2013•Journal of Zhejiang Ocean UniversityRequires access
Isolation and Characterization Microsatellite Markers in Portunus trituberculatus
Yong‐Bing Xiang
Open publisher page 0 citations
Abstract
In this study, CT/AG microsatellite markers were separated using 5'anchored primer PCT in swimming crab genomic DNA. Among the 41 sequenced colons, 35(85.4%) sequences have microsatellites. Apart from some replications, 55 microsatellites were searched in 20 sequences. And 19 sequences have more than one microsatellite. The classifying for the repeat types of these microsatellites performed that among the 55 microsatellites, 27 microsatellites(50%) were CT/AG type and 27 were other types. The results showed that CT/AG microsatellite was abundant in P. trituberculatus; Additionally some other type microsatellites were isolated at the same time using 5'anchored primer. The selection efficiency was raised significantly.