Comparsion of immunological efficacy of two methods of H1N1 influenza vaccine

Objective To compare of immunological efficacy of H1N1influenza vaccine 0done-dose method and 0, 21d,two-dose method,and observe whether seasonal influenza vaccine has cross protection for H1N1influenza virus.Methods H1N1influenza vaccine with two immunological methods was used to inoculate Balb/c mice.The first,the Balb/c mice were inoculated in 0d.The second,the Balb/c mice were inoculated in 0and 21d.The serum from orbital in 14,21,28,35,42dafter the first-dose were separated.HI was performed to determine the H1N1-specific serum antibody titers to compare the immune effect of the two immunological methods.Then the Balb/c mice were inoculated with seasonal influenza vaccine.The serum from orbital in 14and 28dwere separated. HI was performed to determine whether the anti-serum of seasonal influenza vaccine could non-specificly bind with H1N1influenza virus-specific antigens.Results The 28,35and 42dantibody titers of the two-dose method were significantly higher than those of one-dose methods after the second immunity at the 21th day.And the 42d antibody titers of the two-dose methods were 4times of that of one-dose methods.HI was performed to determine the anti-serum of seasonal influenza vaccine,there were is no H1N1influenza virus-specific antibodis in it. Conclusion The antibody titers of 0and 21dtwo-dose method still maintain the high level at 28th day,and are obviously higher than those of one-dose methods.There is no cross-protective immunity betweenseasonalinfluenzavaccine and influenza A H1N1influenza virus.