Construction of Eukaryotic Expression Recombinant Plasmids of VP Gene of Avian Encephalomyelitis Virus

Three pairs of primer were designed according to avian encephalomyelitis virus(AEV) isolate NH937.The structural protein VP1,VP3 and VP0 genes of AEV were amplified by RT-PCR.These genes were cloned into the plasmid pUCm-T,and the inserts were sequenced.The result of nucleotide sequencing showed VP1,VP3 and VP0 genes consisted of 810 bp,735 bp,726 bp and encoded 270,245,242 amino acids,respectively.The VP1,VP3 and VP0 genes of AEV-NH937 strain shared respectively 90.62%,93.88%,95.45% homology with those from the Calnek vaccine strain of AEV,and that deduced amino acids sequences from the VP1,VP3,VP0 genes of AEV-NH937 strain shared respectively 88.89%,97.96%,98.35% homology with those from the Calnek vaccine strain of AEV.To express VP1,VP3,VP0 in E.coli,three recombinant expression vector were constructed and transformed into E.coli,then incubated at 37℃.