Analysis of Rh-positive from blood type antibody screening in 43 pregnant women

Objective:To research on the relationship of Rh blood type antibody in multiple pregnancy and hemolytic disease of the newborn.Method:Rh antibody and subgroup were identified by microcolumn gel immunization assay,and antibody titer was detected by anti-globulin test.Result:Among 16845 pregnant women,48 cases had abnormal antibody,including 43 women with Rh antibody and ciscompatible with husband ABO blood type.From the analysis of antibody subtype and titer,8 women were anti-D,with titer from 8 to 512.5 women were antic,with titer from 16 to 128.16 women were anti-E,with titer from 8 to 64.12 women were anti-C,with titer from 4～8.2 were antie,with titer are 8.And the antigenicity of Rh blood type was DcECe.Conclusion:The titer of Rh subantibody in multiple pregnant women might be one of the reasons for the development of hemolytic disease in the newborn.