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2003•Acta Acadimae Qingdao UmvesitatisRequires access

THE EFFECT OF UVB ON APOPTOSIS OF HaCaT CELLS

Ji Chen feng

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Abstract

Objective To investigate the effect of UVB on apoptosis of HaCaT cells and its mechanism. Methods Cultured HaCaT cells were exposed to 30 mJ/cm 2 UVB and subsequently incubated for 18 hours. A flow cytometry was used to measured apoptotic rate, mitochondrial membrane potential and intracellular free Ca 2+ . Ultrastructure of HaCaT cells was observed through transmission electron microscope. Results UVB irradiation enhanced the apoptotic rate of HaCaT cells ( t= 5.236 , P 0.01), reduced mitochondrial membrane potential ( t=6.897, P 0.01) and elevated the level of intracellular free Ca 2+ ( t=6.064, P 0.01). The cellular ultrastructure underwent severe damage due to UVB irradiation. Conclusion UVB is able to induced apoptosis of HaCaT cells. Reduction of mitochondrial membrane potential and elevation of intracellular free Ca 2+ are involved in the process.

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Objective To investigate the effect of UVB on apoptosis of HaCaT cells and its mechanism. Methods Cultured HaCaT cells were exposed to 30 mJ/cm 2 UVB and subsequently incubated for 18 hours. A flow cytometry was used to measured apoptotic rate, mitochondrial membrane potential and intracellular free Ca 2+ . Ultrastructure of HaCaT cells was observed through transmission electron microscope. Results UVB irradiation enhanced the apoptotic rate of HaCaT cells ( t= 5.236 , P 0.01), reduced mitochondrial membrane potential ( t=6.897, P 0.01) and elevated the level of intracellular free Ca 2+ ( t=6.064, P 0.01). The cellular ultrastructure underwent severe damage due to UVB irradiation. Conclusion UVB is able to induced apoptosis of HaCaT cells. Reduction of mitochondrial membrane potential and elevation of intracellular free Ca 2+ are involved in the process.

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Available abstract

Objective To investigate the effect of UVB on apoptosis of HaCaT cells and its mechanism. Methods Cultured HaCaT cells were exposed to 30 mJ/cm 2 UVB and subsequently incubated for 18 hours. A flow cytometry was used to measured apoptotic rate, mitochondrial membrane potential and intracellular free Ca 2+ . Ultrastructure of HaCaT cells was observed through transmission electron microscope. Results UVB irradiation enhanced the apoptotic rate of HaCaT cells ( t= 5.236 , P 0.01), reduced mitochondrial membrane potential ( t=6.897, P 0.01) and elevated the level of intracellular free Ca 2+ ( t=6.064, P 0.01). The cellular ultrastructure underwent severe damage due to UVB irradiation. Conclusion UVB is able to induced apoptosis of HaCaT cells. Reduction of mitochondrial membrane potential and elevation of intracellular free Ca 2+ are involved in the process.

Key concepts: HaCaT, Apoptosis, Intracellular, Mitochondrion, Flow cytometry, Ultrastructure, Cell biology, Membrane potential

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