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Mapping QTLs and Epistasis for Seedling Vigor in Rice(Oryza sativa L.)

Cao Li

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Abstract

A double haploid (DH) population derived from across between a japonica male parent Azucena and indica female parents IR64, was used for detecting QTLs with additive effects and their epistasis for seedling vigor related traits of rice. Under low temperature and moderate temperature germination condition, the length of mesocotyl, celeoptile, shoots and roots of 109 DH lines were measured. QTLs analysis was conducted by the software QTL mapper. Four QTLs with additive effects and two pairs of QTLs with epistatic effects controlled mesocotyl length. There are nine QTLs with additive effects and five pairs of QTLs with epistatic for celeoptile length, six QTLs with additive effects and six pairs of QTLs with epistatic for root length, five QTLs with additive effects and four pairs of QTLs with epistatic effects for shoot length, respectively. Some additive×environment and epistasis×environment interaction effects of QTLs for seedling vigor related traits are also detected.

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What this paper is about

A double haploid (DH) population derived from across between a japonica male parent Azucena and indica female parents IR64, was used for detecting QTLs with additive effects and their epistasis for seedling vigor related traits of rice. Under low temperature and moderate temperature germination condition, the length of mesocotyl, celeoptile, shoots and roots of 109 DH lines were measured. QTLs analysis was conducted by the software QTL mapper. Four QTLs with additive effects and two pairs of QTLs with epistatic effects controlled mesocotyl length. There are nine QTLs with additive effects and five pairs of QTLs with epistatic for celeoptile length, six QTLs with additive effects and six pairs of QTLs with epistatic for root length, five QTLs with additive effects and four pairs of QTLs with epistatic effects for shoot length, respectively. Some additive×environment and epistasis×environment interaction effects of QTLs for seedling vigor related traits are also detected.

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Available abstract

A double haploid (DH) population derived from across between a japonica male parent Azucena and indica female parents IR64, was used for detecting QTLs with additive effects and their epistasis for seedling vigor related traits of rice. Under low temperature and moderate temperature germination condition, the length of mesocotyl, celeoptile, shoots and roots of 109 DH lines were measured. QTLs analysis was conducted by the software QTL mapper. Four QTLs with additive effects and two pairs of QTLs with epistatic effects controlled mesocotyl length. There are nine QTLs with additive effects and five pairs of QTLs with epistatic for celeoptile length, six QTLs with additive effects and six pairs of QTLs with epistatic for root length, five QTLs with additive effects and four pairs of QTLs with epistatic effects for shoot length, respectively. Some additive×environment and epistasis×environment interaction effects of QTLs for seedling vigor related traits are also detected.

Key concepts: Epistasis, Doubled haploidy, Quantitative trait locus, Biology, Japonica, Seedling, Oryza sativa, Japonica rice

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