Distribution and excretion of muscovy duck reovirus attenuated strain in vaccinated ducklings

One-day-old muscovy ducks were immunized with muscovy duck reovirus attenuated strain by the route of muscle injection,then the viruses in blood,heart,liver,spleen,lung,kidney,pancreas,larynx and cloaca were detected by RT-PCR to explore the distribution and excretion in the vaccinated ducklings.The results indicated that the tissue of blood,heart,liver and spleen were positive 4 h post vaccinated(PV);lung,kidney and pancreas were positive 8 h post vaccinated;larynx and cloaca were positive 3 d post vaccinated but were negative 14 d post vaccinated;kidney and pancreas were negative 28 d post vaccinated;blood and heart were negative 42 d post vaccinated;all tissues were negative 49 d post vaccinated.Above all,the sustaining time of muscovy duck reovirus attenuated strain was 4 h-35 d in blood and heart,4 h-42 d in liver and spleen;8 h-25 d in lung,kidney and pancreas.The excretion time was 3-14 d.