Study of toxicity of Aβ_(25～35) on cultured vascular smooth muscle cells of aorta

Objective To investigate the toxicity of amyloid-beta protein 25～35(Aβ25～35) on cultured vascular smooth muscle cells in vitro. Methods The control group was normal saline. In Aβ25～35 group, VSMC were incubated with Aβ25～35 at different concentrations(10?20?50?100 μmol/L) for 1d, 3 d and 10 d. VSMC morphology was detected with light microscope, vitality was measured by MTT method, and the lactate dehydrogenase concentration was tested. Results After incubated with 20 μmol/L Aβ25～35 for 3d, the survival rate was decreased and the lactate dehydrogenase level was increased and VSMC began to degenerate. The degree of cell degeneration, decreasing of survival rate and increasing of lactate dehydrogenase concentration all showed Aβ25～35 concentration and incubation time dependence. Conclusions The toxicity of Aβ25～35 on VSMC includes changing of cell morphology, decreasing survival rate and increasing lactate dehydrogenase level.