Preparation of Purified Bivalent Haemorrhagic Fever with Renal Syndrome Vaccine

Objective To prepare purified bivalent haemorrhagic fever with renal syndrome(HFRS)vaccine containing virus antigens types Ⅰ and Ⅱ and improve its quality and immunogenicity.Methods Prepare bivalent HFRS vaccine by mixing the PS-6(type Ⅰ) and L99(type Ⅱ) strains cultured in primary hamster kidney cells(PHKC)at a volume ratio of 1∶1,and purify by precipitation with zinc acetate,concentration with ultrafiltration and Sepharose 4FF column chromatography.Results All the indexes of the purified bivalent HFRS vaccine were qualified in control tests in the NCL.The vaccine was qualified in potency test after storage at 37℃ for 2 weeks or at 4℃ for 3 years.The positive conversion rate of neutralizing antibody induced by the vaccine was above 85%,and only mild adverse reactions were observed in 5% of recipients.Conclusion The prepared vaccine met the (Trial)Requirements for Purified Bivalent Haemorrhagic Fever with Renal Syndome Vaccine and showed good quality and clinical effect.