Pharmacokinetic study of ferulic acid in Ruxian Pill in rabbits in vivo

Objective To study the pharmacokinetics of ferulic acid in rabbits in vivo after ig administering Ruxian Pill.Methods The concentrations of ferulic acid in plasma were determined by HPLC.Taking coumarin as internal standard,a mixture of methanol-acetic acid-water(38.0∶0.5∶61.5) was used as the mobile phase,and UV detection was performed at 320 nm.The analytical column was ODS Hypersil C_(18)(150 mm×4.6 mm,5 μm).Results The calibration curve was linear over the range of 12.5—1 600.0 μg/L and the measurable limit of plasma detection was 6.25 μg/L.The recovery was 96.17%,99.80%,100.23%. The RSD of the intra-day and the inter-days was less than 6%.The plasma concentration-time curve was consistent with one-comparment model.The pharmacokinetic parameters were as follows: AUC_(0-4 h)(855.29±100.78) μg/(L·h),AUC_(0-∞)(1 051.99±125.96) μg/(L·h),C_(max)=(952.71±119.04) μg/(L·h),t_(max)=(0.5±0.00) h,t_(1/2)=(1.61±0.21) h,K_e=(0.46±0.05)/h. Conclusion The method is accurate and precise with better reproducibility,which can be used for the determination of plasma level of ferulic acid and the study of its pharmacokinetics for Ruxian Pill.