Change of p21 after partial hepatectomy in rats with non-alcoholic fatty liver

Objective To investigate the changes of p21 after the partial hepatectomy in rats with non-alcoholic fatty liver disease(NAFLD).Methods The rat models of non-alcoholic fatty liver disease were established by giving a fat-riched diet.The other rats fed with normal diet were taken as normal control at the same stage during the study.These rats were sacrificed at 0,1,12,24,36h respectively after 70% partial hepatectomy at 12 week and the liver tissues were collected.The RT-PCR and Western blot were used to dectect the change of p21.The positive cell percentage of proliferation cell nuclear antigen(PCNA) was measured by immunohistochemical method.Results The liver regenerative model of rats with non-alcoholic fatty liver disease was estabilished successfully.As compared with the control group the expression of p21 in F group was higher than that of C group at both mRNA and protein level.The positive cell percentage of PCNA was lower than that of C group.Conclusion The expression of p21 in rats with NAFLD after partial hepatectomy was higher than that in control group at both mRNA and protein level.At the same time the regenerative capaticity of liver in F group was decreased.