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[Effects of genistein on proliferation and differentiation of osteoblast].

Yuehong Zhang, Lanxing Gao, Hong Jin, Zhiqin Xu, Nan Wen-kao, Xianyuan Wang

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Abstract

AIM: To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures. METHODS: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit. RESULTS: Genistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity. CONCLUSION: Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.

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What this paper is about

AIM: To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures. METHODS: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit. RESULTS: Genistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity. CONCLUSION: Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.

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Available abstract

AIM: To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures. METHODS: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit. RESULTS: Genistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity. CONCLUSION: Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.

Key concepts: Genistein, Calvaria, Osteoblast, Chemistry, Collagenase, Endocrinology, Internal medicine, MTT assay

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