2007•PubMedRequires access
[Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin].
Lifang Huan, Lihong Yao, Aijun Chen, Cong-Sheng Cheng, Runqing Jia, Qing Tian, Bo Hong, Jianqiang Guo, Min Wang, Yuelong Shu, Zhiqing Zhang
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Abstract
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.