Change of expression of the androgen receptor coactivator DDC in the effect of flutamide on prostate cancer cell LNCaP

Objective To investigate the expression of androgen receptor coactivator DDC in the effect of flutamide on the prostate cancer cell line-LNCaP.Methods MTT and flow cytometer were employed to explore the effect of flutamide on LNCaP,and cDNA microarrays were performed to elucidate DDC's expression in this process.Results The LNCaP cells' growth accelerated within 7 days after hydroxyflutamide treatment.However,it was inhibited after 5 generations.And this inhibition was correlated with the dosage treated.The cell cycle was blocked mainly in G1 phase.The correlated molecular mechanism was the down-regulation of the androgen receptor coactivator DDC.Conclusion Hydroxyflutamide could stimulate LNCaP cell within several days.However,with the drug-treating time prolonging,the stimulation effect switched to inhibition,which is due to the inhibition of DDC's expression,influencing the expression of its correlated downstream cell cycle related genes,thus blocking cell cycle in G1/S checkpoint.