Screen and acclimation tolerant to methanol of strains producing lipase

The lipase-producing strains were isolated from soils.Six strains out of the 34 isolates were selected by the larger transparent zone on the selective medium with victoria blue.Strain FM-1 was the best strain producing intracellular lipase with high activity 132 U/g cell.The strain FM-1 was acclimated repeatedly by the traditional cultivation in the medium containing mehanol,which became the strain tolerant to methanol.After acclimation in the medium containing 11 mg/L methanol,the relative lipase activity produced by strain was 35%,the original strains could not grow under the same condition.