The Sequence and Phylogenetic Analysis of 18S rDNA from Eimeria magna

The oocyst of Eimeria magna was isolated and purified from a rabbit farm in Hebei.Its genomic DNA was extracted by CATB.The 18S rDNA gene fragment of E.magna was amplified using conservative primer of 18S rDNA of Eimeria and sequenced.Then it was analysed by DNAStar program package and was aligned with corresponding sequence of other eleven species of rabbit-infecting Eimeria in the GenBank.And then the phylogenetic tree was establised.The results indicated that the amplified gene fragment was about 1 522 bp.The sequence analysis showed that the genetic homology between the 18S rDNA of E.magna isolated from Hebei and the corresponding sequence of E.magna publicized in GenBank was most close and the similarity was up to 99.6%;and the monology between Hebei E.magna and the other 11 kinds of Eimeria infecting overseas rabbit was 92.4%~99.6%.