Applicability of microsatellite DNA markers in parentage determination of Portunus trituberculatus

Based on four families obtained by directional mating techniques,the application of two pairs of microsatellite markers,Pot17 and Pot42,in Portunus trituberculatus parentage determination was explored. Eleven alleles were found at two microsatellite DNA loci (six at locus Pot17 and five at locus Pot42 respectively) by investigating 5 parents and 98 offsprings in the four families. The analysis results showed that the gene frequencies detected by locus Pot17 and Pot42 were between 0.029 1 and 0.359 2,expected heterozygosity was 0.774 0 at locus Pot17 and 0.757 7 at locus Pot42,observed heterozygosity was 0.621 4 at locus Pot17 and 0.679 6 at locus Pot42,and the polymorphism information content (PIC) was 0.737 3 at locus Pot17 and 0.712 9 at locus Pot42,respectively. The results showed that the cumulate power of discrimination (CDP) of Pot17 and Pot42 reached up to 0.995. They are applicable in the Portunus trituberculatus parentage determination.