Effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside on expression of vimentin in aorta of atherosclerotic rats

Aim To investigate the mechanisms of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside( TSG)for its atherosclerotic effects,and screen novel targets of TSG. Methods Rats were fed with high fat diet and ip vitamin D3to establish atherosclerosis( AS)model. Rats were divided into the following groups:control group( normal feeds),model group( high fat feeds),TSG 30 mg·kg- 1·d- 1,TSG 60 mg·kg- 1·d- 1,TSG 120 mg·kg- 1·d- 1. After 12 weeks,aortas of rats were separated. Total protein and RNA were extracted. Differential proteins were analyzed by two-dimensional gel electrophoresis( 2-DE) and quantified initially. Differential proteins were excised and analyzed by enzymolysis and mass spectroscopy( MS).Expression and transcription of vimentin were detected by Western blot and RT-PCR analysis,respectively.Results 2-DE and MS analysis indicated that the differential protein after TSG treatment was vimentin.When compared with the control group,expression of vimentin in AS model group was greatly increased. After treated with TSG,vimentin expression was declined evidently. Furthermore, according to the results of Western blot and RT-PCR,the inhibitory effect of TSG on vimentin expression might be in the transcription level. Conclusion TSG which could inhibit atherosclerosis may be associated with the down- regulation of vimentin expression. Vimentin is expected to become a novel TSG target.