Expression of microRNA-200a in hepatic carcinoma cell line HepG2

Objective To investigate the expression of miR-200a in hepatic cancer cell line HepG2 and its effect on biological features of hepatic carcinoma.Methods The expression of miR-200a was quantitatively examined by RT-PCR in HepG2 cells and normal liver QSG7701 cells.miR-200a mimics were transfected into HepG2 cells,the cell proliferation was assessed by MTT method,cell cycle and apoptosis were examined by flow cytometry and the migration of cells was assayed by Transwell test.Results The relative expression of miR-200a in HepG2 cells was 4.79 times of that in QSG-7701 hepatic cells(P0.01).MTT assay revealed that 150nmol/L miR-200a mimics significantly promoted proliferation of HepG2 cells at 72h after transfection with a promotion rate of 38.4%.Compared with controls,there was no significant changes in percentage of Go/G1 phase(57.13±0.02)%,S phase cells(26.55±0.8)% and the proportion of early apoptotic cells(0.5±0.06)% of HepG2 cells after miR-200a mimics transfection(P0.05).There were significant differences in cell migration between HepG2 cells after transfected with 100 nmol/L miR-200a and two control groups(0.348+0.005657 vs 0.296+0.006364 and 0.287+0.005364,P0.05).Conclusion miR-200a expression in HepG2 cancer cells is up-regulated,which may be associated with hepatic cancer cell proliferation and invasion.