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2010•Chinese Journal of Difficult and Complicated CasesRequires access

Expand culture of rat bone marrow endothelial progenitor cell in vitro

Wang Hao-wen

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Abstract

Objective To investigate the method for establishing endothelial progenitor cell(EPC) in vitro. Methods The mononuclear cells separated from rat bone marrow by density gradient separation.After 7 days'culture, cells were stained with Dil-acLDL and FITC-UEA-1,and examined by flow cytometry,tested the abilities of migration. Results The spindle cells grew dominantly after 7 days' culture,the percentage of cells labeled both with Dil-acLDL and FITC-UEA-1 was(82.7±5.1)%,the slab stone like colony appeared in 10 days,the cells positive for CD34 in7 days and 14 days were(32.3±3.2)%,(39.2±4.4)%(t=5.18,P0.01),respectively.The cells positive for FLK-1 was(28.7±3.2)%,(44.5±3.8)%(t=5.32,P0.01),respectively.The number of migratory cells in day 7 and day 14 were(10.4±1.9) and(11.8±2.5)(t=0.83,P0.05),respectively.Conclusion The method in this study is a good choice for separating and assessing EPC in rat bone marrow.

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What this paper is about

Objective To investigate the method for establishing endothelial progenitor cell(EPC) in vitro. Methods The mononuclear cells separated from rat bone marrow by density gradient separation.After 7 days'culture, cells were stained with Dil-acLDL and FITC-UEA-1,and examined by flow cytometry,tested the abilities of migration. Results The spindle cells grew dominantly after 7 days' culture,the percentage of cells labeled both with Dil-acLDL and FITC-UEA-1 was(82.7±5.1)%,the slab stone like colony appeared in 10 days,the cells positive for CD34 in7 days and 14 days were(32.3±3.2)%,(39.2±4.4)%(t=5.18,P0.01),respectively.The cells positive for FLK-1 was(28.7±3.2)%,(44.5±3.8)%(t=5.32,P0.01),respectively.The number of migratory cells in day 7 and day 14 were(10.4±1.9) and(11.8±2.5)(t=0.83,P0.05),respectively.Conclusion The method in this study is a good choice for separating and assessing EPC in rat bone marrow.

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Available abstract

Objective To investigate the method for establishing endothelial progenitor cell(EPC) in vitro. Methods The mononuclear cells separated from rat bone marrow by density gradient separation.After 7 days'culture, cells were stained with Dil-acLDL and FITC-UEA-1,and examined by flow cytometry,tested the abilities of migration. Results The spindle cells grew dominantly after 7 days' culture,the percentage of cells labeled both with Dil-acLDL and FITC-UEA-1 was(82.7±5.1)%,the slab stone like colony appeared in 10 days,the cells positive for CD34 in7 days and 14 days were(32.3±3.2)%,(39.2±4.4)%(t=5.18,P0.01),respectively.The cells positive for FLK-1 was(28.7±3.2)%,(44.5±3.8)%(t=5.32,P0.01),respectively.The number of migratory cells in day 7 and day 14 were(10.4±1.9) and(11.8±2.5)(t=0.83,P0.05),respectively.Conclusion The method in this study is a good choice for separating and assessing EPC in rat bone marrow.

Key concepts: Bone marrow, CD34, Progenitor cell, Peripheral blood mononuclear cell, In vitro, Medicine, Flow cytometry, Andrology

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