Laboratory Application of PCR for the Identification of Sporothrix Scheckii

Objective To study a rapid method to detect and identify Sporothrix schenckii by using Species-specific oligonucleotide primer PCR techniques,and lay the fundation of molecular diagnosis for sporotrichosis.Methods The Species-specific oligonucleotide primer pair S2-R2 was used in this study.It was designed from nucleotide sequences of chitin synthase 1 gene in Sporothrix schenckii.26 strains of Sporothrix schenckii,6 strains of clinical common fungi were amplified by PCR.26 strains of Sporothrix schenckii include 1 standard strain,7 strains of laboratory reserved and 18 strains of clinical isolated.Results All strains of Sporothrix schenckii showed a specific fragment of 318bp with the species-specific primer pair.Using the same primer pairs,the others species had no specific amplification.Conclusion This method is specific,rapid and simple for identifying Sporothrix schenckii and could be used for clinical molecular diagnosis.