Effence of verapamil in chemotherapy of rats hepatocarcinma of Hca-A2/p

[Objective] :To offer basic theory for liver carcinoma treatment by observing the therapeutic effects of verapamil and chemotherapeutic agent in loading tumor rats. [Methods] Pharmaceuticals were injected in tumor of loading tumor rats in different points for many times, and then ,to observe Inhibition rate of tumor、pathology of tumor and rats of mortality and to determine expression of multidrugs resistance gene (mdr1) by RT-PCR. [Results] Expression of mdr1 in loading tumer group(LT)c、hemiotherapy group(C)、Verapamil group (V)、 chemotherapy Verapamil group (CV) is ( 13/14、14/14、3/14、1/14) respectively and there is statistical signifi- cance between V group(or CV group) and LT group,P0.05; between LT++ group and C++ group,P 0.05. Inhibition rate of tumor of C group、CV group is (35%,63.6%)respectively ,which compare with LT group,P0.05;CV group compare with V group in inhibition rate of tumor,P0.05. Rats’mortality in C group(3/20)、CV group( 2/20)compare with LT group(8/20),P0.05. Tumor’s necrosis is outstanding in CV group. [Conclusion] Applying verapamil and chemotherapeutic agent together in loading rats have more ef- fect than only chemotherapeutic agent .It indicate that reversing drugs of mdr1 and chemotherapeutic agent be applied at the same time can better therapy effects of liver carcinoma.