Detection of Th1/Th2 Cytokines in the Peripheral Blood Lymphocytes of Patients with Condyloma Acuminatum

Objective Methods Results Conclusion >To detect cytokine levels secreted by CD3+T cells and monitor the Th cell imbalance in the patients with condyloma acuminatum(CA).Methods The peripheral blood lymphocytes were isolated and stimulated prior to analysis.Cytokine detection was performed using monoclonal antibodies,which were fluorochrome-conjugated for easier direct manipulation in flow cytometry analysis.The results were pre-sented as cytograms showing dual staining of lymphocytes.Results The levels of IL-2,IL-12,IFN-γ and TNF-α produced by Th1cells decreased,while the level of IL-10 produced by Th2 cells had no significant change in CA patients in comparison with healthy controls.Conclusions There is Th1/Th2 imbalance in CA patients with a decline of Th1type cytokines.The Th1/Th2 imbalance might be the mechanism that HPVs escape from immunosurveillance in CA patients. Key words: Condylomata Acuminata; Warts