The Effects of RNA Interference to c-Met Gene on Cytobiology Behavior of Human Melanoma Cell Line A375

Objective To investigate the effects of RNA interference to c-Met gene on proliferation and invasion of human melanoma cell line A375. Methods The pGenesil-1 plasmid was used to constructed shRNA of c-Met and was transfected into A375 cells. Morphological changes of transfected cells were observed under inversed microscopy. The mRNA and protein level of c-Met were detected by RT-PCR and Western blot. The proliferating and invading ability of transfected A375 cells were evaluated by MTT and Transwell cabin separately. Results Transfected cells with pGenesil-1-c-Met shRNA showed round and small. Results of RT-PCR and western blot revealed c-Met mRNA was remarkably decreased,especially 48h later was decreasing nearly 64% while level of c-Met protein decreased nearly 65% after transfection. MTT confirmed c-Met shRNA could suppress the growth of A375 cells to(68.25±4.4)%. The invasion of A375 cells were significantly decreased. Conclusion pGenesil-1-c-Met shRNA could significantly inhibit c-Met expression,suppress the growth of A375 cells and decrease their invasion.