Biochemical Genetic Markers of the Inbred Strain C1HD of Carassius auratus Red Variety

Objective To set up biochemical genetic markers of the inbred strain C1HD of Carrassius aurratus red variety as a laboratory animal.Methods MDH,SOD and serum proteins of both the inbred strain C1HD of Carassius auratus red variety and the wild ones were analyzed by polyacrylamide gradient gel vertical electrophoresis. Results More than twenty-five bands of serum proteins from the two types of Carassius auratus red variety were separated,which could be divided into A,B and C regions.There were 14 bands at the region C near the anode,while an apparent SP-A1 band at region A near the cathode was detected in the inbred strain C1HD of carassius auratus red variety,but SP-A2 and SP-A3 were not detected.There were 8 bands at the region B,while SP-B2 was not detected in the inbred strain C1HD of Carassius auratus red variety.There were three MDH isozyme electrophoretic bands of inbred strain C1HD of Carassius auratus red variety(MDH-1,MDH-2,MDH-3) and those were five(MDH-1,MDH-2,MDH-3,MDH-4,MDH-5) in the wild ones. There were eight SOD isozyme electrophoretic bands of the inbred strain C1HD of Carassius auratus red variety and six in the wild ones,and the former have two more bands(SOD-3,SOD-8) than the latter,but the respective of the two fishes are the same.Conclusion SP-A1,SOD-3 and SOD-8 of electrophoretic bands can be used as biochemical genetic markers of the inbred strain C1HD of Carassius auratus red variety.